A New Model for Growth and Filament Production of Pityrosporum Ovale (Orbiculare) on Human Stratum Corneum In Vitro
نویسندگان
چکیده
منابع مشابه
An In vitro Model for Bacterial Growth on Human Stratum Corneum.
The diversity and dynamics of the skin microbiome in health and disease have been studied recently, but adequate model systems to study skin microbiotas in vitro are largely lacking. We developed an in vitro system that mimics human stratum corneum, using human callus as substrate and nutrient source for bacterial growth. The growth of several commensal and pathogenic bacterial strains was meas...
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Reinhold Dauskardt Stanford University, Professor Materials Sciences and Engineering The stratum corneum (SC) is the outermost layer of the skin. Its process of reneweal is regulated by various mechanisms, including chemical and structural gradients through the SC thickness. In this study, an in vitro mechanics approach for studying the delamination behavior of SC in the direction perpendicular...
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Bioptic specimens from 224 melanocytic naevus lesions and 121 seborrheic keratosis lesions were stained with hematoxylin-eosin and examined under the light microscope. Pityrosporum ovale/orbiculare (Malassezia furfur) was found in 35% of melanocytic naevi and in 36% of seborrheic keratoses. The finding of Pityrosporum ovale/orbiculare was confirmed by PAS (periodic acid shift), methenamide silv...
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Allergic contact dermatitis due to nickel salts is common. It is therefore important to measure the permeation of these salts through the stratum corneum (SC), the primary rate-limiting domain in skin. An advanced diffusion system and analytical techniques now enable better measurement of the flux than was possible in earlier experiments. Human SC was prepared by trypsinization of dermatomed ca...
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The primary function of the skin is to act as a barrier against unwanted influences from the environment. The barrier function of the skin is located in the superficial of the skin, the stratum corneum. The stratum corneum consists of dead cells filled with keratin and water, which are embedded in lipid regions. The lipid regions are the only continuous structure in the stratum corneum. For thi...
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 1989
ISSN: 0022-202X
DOI: 10.1111/1523-1747.ep13071328